Part:BBa_M10052

{˂ehaB!}

The EhaB part codes for an autotransporter protein that can transport itself across the bacterial membrane and go to the cell surface. Like other autotransporter proteins, the EhaB part has an N-terminal signal sequence, a passenger domain, and a translocation domain. EhaB expressed in E. Coli MS427 has been shown to form biofilms on polystyrene surfaces. Also, overexpression of the protein in E. Coli K-12 has resulted in biofilm formation under continuous flow conditions.
The EhaB protein expression in E. Coli K12 and EDL933 was shown to promote adhesion to the extracellular matrix proteins collagen I and laminin. Moreover, EhaB can be expressed in vivo and can elicit an IgA immune response.

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This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 589
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 589
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 589
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 589
  Illegal AgeI site found at 132
  
• 1000
  COMPATIBLE WITH RFC[1000]

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